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The scanner-specific DTI acquisition parameters are listed in Table 4. In order to minimize head motion of the subject for a long period of scan time, three or four foam blocks were inserted between the head and the head coil to hold the head still. Data from all vendors were first inspected visually for the presence of image volumes with missing slices or large motion artifacts prior to processing.
All subsequent analyses were done using internally developed software written in IDL 8. Mean signal intensity and noise were assessed from the average and subtraction of two magnitude images of consecutive acquisitions, respectively.
In construction of image sets with a specific NSA, each acquisition was used at least once. Using software written in IDL 8. Additionally, areas from two adjacent slices were combined to form an ROI in order to further increase the ROI volume. Thus, the ROI-based approach can decrease the noise due to the spatial signal averaging and also diminish the uncertainty of the outcomes. The ROIs were drawn on the color-coded FA map where the selected regions of the brain had different colors due to distinct fiber orientations Supplementary Fig.
The ROI size was calculated as follows:. First the center of the area in each slice was calculated. Then the long axis of the area was found as the line connecting any two points in the slice inside the ROI with the longest distance. The short axis of the area was defined as the line perpendicular to the long axis and passing the center of the area. The short axis divided the area into two sub-ROIs in the slice.
For each diffusion tensor, the direction of the eigenvector with the largest eigenvalue was considered as the primary diffusion direction. If the probability of incorrectly rejecting the null hypothesis of difference between two FA values was less than 0.
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Termination criteria may be based on FA value terminate tracking when FA falls below a certain value, e. Anyone who plays around with setting seed locations and termination parameters can easily manipulate visual displays of fiber tracts.
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